def getMatrix(dirname):
    pssmList = os.listdir(dirname)
    pssmList.sort(key=lambda x: eval(x[:]))
    m = len(pssmList)
    reMatrix = np.zeros((m, 20))
    for i in range(m):
        matrix= readToMatrix(dirname + '/' + pssmList[i], 'pssm')
        matrix = autoNorm(matrix, 'pssm')
        reMatrix[i, :] = get20Aminos(matrix)
    print(reMatrix.shape)
    return reMatrix

补充

os.listdir
返回指定路径下所有文件和文件夹的名字，并存放于一个列表中。